Nurturing Life Sciences
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#Unit 13. Methods in Biology
Capillary zone electrophoresis is the example of free solution electrophoresis with highest resolution using high voltage and using Electrophoretic mobility (EFM) & Electroendosmosis force (EEOF).
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Capillary zone electrophoresis can analyze hybridization probes, gene-cloning, DNA sequencing, polymerase chain reaction (PCR), HIV load, site-directed mutagenesis, & antisense therapeutics.
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Point mutations in DNA, quantification of DNA, Chiral compounds all ca be analyzed by using Capillary zone electrophoresis.
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Output of capillary zone electrophoresis known as electropherogram.
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Microchip electrophoresis is also based on free flow.
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Slab gels can develop a “Smiling Effect” When in a gel more Joule heat is produced than can be dissipated, leads to the higher mobility of the ions in the center, resulting in an uneven distribution of the zones, These zones bend up at the two lateral sides, causing “smiling.”