The origin of E. coli, oriC, is 245 bp in length. The genome of E. coli is replicated bidirectionally from a single unique site called the origin, identified as the genetic locus oriC.

Two replication forks initiate atoriCand move around the genome at approximately the same speed to a special termination region.

TLS Online TPP Program

#Id: 8310

#Unit 1. Structure and Function of Biomolecules

TLS Online TPP Program

#Id: 8541

#Unit 1. Structure and Function of Biomolecules

The dependent variable is the rate of the reaction. 

The independent variables are the concentration of enzyme, the concentration of the substrate, the temperature, and the addition of an inhibitor

TLS Online TPP Program

#Id: 8542

#Unit 1. Structure and Function of Biomolecules

If the concentration of the enzyme or substrate is increased, the rate of the reaction will be increased as well. 

If the temperature of the enzymes surrounding environment is increased, then the rate of the reaction will also be increased. 

If the substrate concentration is increased then the rate of the reaction will increase. 

If an inhibitor is added then the rate of the reaction will be decreased significantly

TLS Online TPP Program

#Id: 8543

#Unit 1. Structure and Function of Biomolecules

One drawback from the Eadie–Hofstee approach is that neither ordinate nor abscissa represent independent variables: both are dependent on reaction rate. Thus any experimental error will be present in both axes.

TLS Online TPP Program

#Id: 8544

#Unit 1. Structure and Function of Biomolecules

The abscissa refers to the (x) coordinate and the ordinate refers to the (y) coordinate of a standard two-dimensional graph.

TLS Online TPP Program

#Id: 8545

#Unit 1. Structure and Function of Biomolecules

Irreversible inhibitors, such as the organophosphorus and organomercury compounds, cyanide, carbon monoxide and hydrogen sulphide, combine with the enzyme to form a covalent bond.