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TLS Online TPP Program

#Id: 3179

Replication of mtDNA may be stochastic because there is no control over which particular copies are replicated, so that in any cycle some mtDNA molecules may replicate more times than others.


#Unit 3. Fundamental Processes #Extrachromosomal replicons #Part B Pointers
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TLS Online TPP Program

#Id: 571

#Unit 2. Cellular Organization

Constitutive mutations occur at 8 positions in the operator between +5 and +17 called OC
TLS Online TPP Program

#Id: 572

#Unit 2. Cellular Organization

Repressor binds with O1and O3 tonhibit expression of lacZ
TLS Online TPP Program

#Id: 573

#Unit 2. Cellular Organization

Effects of mutations in the three lac operators
All three operators are essential for effective repression.
TLS Online TPP Program

#Id: 574

#Unit 2. Cellular Organization

OC are constitutive because repressor protein is unable to bind; this allows RNA polymerase to have unrestrained access to the promoter. 
TLS Online TPP Program

#Id: 575

#Unit 2. Cellular Organization

The Oc mutations are cis-acting/cis dominant, because they affect only the contiguous set of structural genes.

 
TLS Online TPP Program

#Id: 576

#Unit 2. Cellular Organization

The DNA-binding domain occupies residues 1–59. It contains two a-helices separated by a turn. This is a common DNA-binding motif known as the HTH (helix-turn-helix); the two a-helices fit into the major groove of DNA