Point mutation (i.e., a change in a single base pair) in a proto-oncogene that results in a hyperactive or constitutively active protein product.

TLS Online TPP Program

#Id: 2288

#Unit 3. Fundamental Processes

The effect of temperature on the dissociation of the polymerase–T7 DNA complex. 

TLS Online TPP Program

#Id: 2289

#Unit 3. Fundamental Processes

higher temperature favors tighter binding between RNA polymerase holoenzyme and T7 DNA

TLS Online TPP Program

#Id: 2290

#Unit 3. Fundamental Processes

RNA polymerase holoenzyme binds DNA loosely at first

a. Binds at promoter initially

b. Scans along the DNA until it finds one

TLS Online TPP Program

#Id: 2291

#Unit 3. Fundamental Processes

Isomerization, does not require energy derived from ATP hydrolysis and is instead the result of a spontaneous conformational change in the DNA– enzyme complex to a more energetically favorable form. 

TLS Online TPP Program

#Id: 2292

#Unit 3. Fundamental Processes

Isomerization is essentially irreversible and once complete, typically guarantees that transcription will subsequently initiate .

TLS Online TPP Program

#Id: 2293

#Unit 3. Fundamental Processes

Formation of the closed complex, in contrast, is readily reversible: polymerase can as easily dissociate from the promoter as make the transition to the open complex.