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The maximum absorption of UV by DNA is at a wavelength of 254 nm. Maximum mutagenicity also occurs at 254 nm. 

#Unit 3. Fundamental Processes #Mutation #Part B Pointers
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#Unit 13. Methods in Biology

We perform DNase footprinting by binding the protein to its DNA target, then digesting the DNA–protein complex with DNase. 

When we electrophorese the resulting DNA fragments, the protein binding site shows up as a gap, or “footprint,” in the pattern where the protein protected the 

DNA from degradation.


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#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).

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#Unit 3. Fundamental Processes

Primers start with the sequence pppAG positioned opposite the sequence 3 '-GTC-5' in the template.

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#Unit 13. Methods in Biology

Tritiated (3H) Thymidine uptake used to assess Cell Division based on radioactivity measurement by scintillation counting.

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#Unit 6. System Physiology – Plant

Auxin is the only plant growth hormone known to be transported polarly.  Auxin efflux takes place by PIN proteins

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