Ultraviolet light induces the formation of a cyclobutane ring between adjacent thymines

TLS Online TPP Program

#Id: 4709

#Unit 13. Methods in Biology

In proteins, there are three types of chromophores relevant for UV/Vis spectroscopy:

• peptide bonds (amide bond);

• certain amino acid side chains (mainly tryptophan and tyrosine); and

• certain prosthetic groups and coenzymes (e.g. porphyrine groups such as in haem).

TLS Online TPP Program

#Id: 1578

#Unit 4. Cell Communication and Cell Signaling

Active Wnt signaling leads to the secretion of Notum.

 

TLS Online TPP Program

#Id: 8031

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 2767

#Unit 13. Methods in Biology

We perform DNase footprinting by binding the protein to its DNA target, then digesting the DNA–protein complex with DNase. 

When we electrophorese the resulting DNA fragments, the protein binding site shows up as a gap, or “footprint,” in the pattern where the protein protected the 

DNA from degradation.

TLS Online TPP Program

#Id: 7768

#Unit 1. Molecules and their Interaction Relevant to Biology

Atoms are indivisible particles that can't be created or destroyed in a chemical reaction.