The LexA repressor is inactivated when it catalyzes its own cleavage at a specific Ala–Gly peptide bond.

TLS Online TPP Program

#Id: 791

#Unit 3. Fundamental Processes

A rut site has a sequence rich in C -41% and poor in G preceding the actual site(s) of termination. The sequence corresponds to the 3’ end of the RNA.

 

TLS Online TPP Program

#Id: 792

#Unit 3. Fundamental Processes

C is by far the most common base (41%) and G is the least common base (14%) in rut sites, vary in length.  As a general rule, the efficiency of a rut site increases with the length of the C-rich/G-poor region.

TLS Online TPP Program

#Id: 793

#Unit 3. Fundamental Processes

Rho is a member of the family of hexameric ATP-dependent helicases. Each subunit has an RNA-binding domain and an ATP hydrolysis domain.

TLS Online TPP Program

#Id: 794

#Unit 3. Fundamental Processes

Rho activity is impeded when ribosomes are translating an mRNA.

TLS Online TPP Program

#Id: 795

#Unit 3. Fundamental Processes

The action of rho factor may create link between transcription and translation when a rho-dependent terminator lies soon after a nonsense mutation.

TLS Online TPP Program

#Id: 796

#Unit 3. Fundamental Processes

Nonsense mutation in one gene of a transcription unit was found to prevent the expression of subsequent genes in the unit even though both genes had their own ribosome binding sites, an effect called polarity.