Nurturing Life Sciences
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#Unit 12. Applied Biology
The pH indicator phenol red is used for monitoring the status of the medium. This will change from red at pH 7.2–7.4 to yellow or fuchsia as the pH becomes either acidic or alkaline, respectively.
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Passaging, is the removal of the medium and transfer of cells from a previous culture into fresh growth medium, a procedure that enables the further propagation of the cell line or cell strain.
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Characteristic Growth Pattern of Cultured Cells
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Trypsin is a serine protease that digest the extracellular protein or matrix protein so that cells get free.
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Dissociation of Adherent Cells