Nurturing Life Sciences
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#Unit 3. Fundamental Processes
DnaA is an ATP-binding protein and its binding to DNA is affected depending on whether ATP
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DnaA is the E. coli licensing factor that must be made anew for each round of replication.
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Replication in dam- E. coli depends on its state of methylation.
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In dam- E. coli If the plasmid is methylated, it undergoes a single round of replication, and if it is hemimethylated products accumulate
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The GATC sites at the origin remain hemimethylated for 13 minutes after replication.
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DNA methylation in bacteria allows the DNA mismatch recognition machinery to distinguish the old template strand from the new strand.