Each nucleosome added to a covalently closed circular template changes the linking number of the associated DNA by approximately –1.2.

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#Id: 10284

#Unit 6. System Physiology – Plant

K+in channels are inhibited directly by the rise in [Ca2+]cyt. Overall, therefore, the increase in [Ca2+]cyt causes the net efflux of anions and K+ from the cell, leading to a fall in guard cell turgor.

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#Id: 4618

#Unit 13. Methods in Biology

 If the antibody preparation is polyclonal and has a range of affinities, Scatchard plot yields a curved line whose slope is constantly changing. The average affinity constant K0 can be calculated by determining the value of Ka when half of the binding sites are occupied (i.e., when r  1 in this example). In this graph, antiserum #3 has a higher affinity (K0  2.4  108) than antiserum #4 (K0  1.25  108)

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#Id: 2751

#Unit 13. Methods in Biology

AFLP uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

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#Id: 10236

#Unit 6. System Physiology – Plant

DE-ETIOLATED2 (DET2) gene, which encodes a brassinosteroid biosynthetic gene. Det2 loss-of-function mutants have reduced levels of brassinosteroids, resulting in a de-etiolated appearance of the seedling even when grown in the dark

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#Id: 6818

#Unit 8. Inheritance Biology

Quantitative character - Large number of possible phenotypes that are not easily distinguished.