By maintaining the unbound DNA in a relaxed state, topoisomerases facilitate the binding of histones to the DNA and the formation of additional nucleosomes.

Importantly, the overall effect on the plasmid is that the linking number is decreased as more nucleosomes are assembled

TLS Online TPP Program

#Id: 798

#Unit 3. Fundamental Processes

rho mutants are usually leaky

TLS Online TPP Program

#Id: 799

#Unit 3. Fundamental Processes

Mutations in the rpoB gene can reduce termination at a rho-dependent site.  Mutations in rpoB can restore the ability to terminate transcription at rho-dependent sites in rho mutant bacteria.

TLS Online TPP Program

#Id: 800

#Unit 3. Fundamental Processes

Transcription is formation of RNA from genome (DNA/RNA)

TLS Online TPP Program

#Id: 801

#Unit 3. Fundamental Processes

On the basis of Transcription, viruses have been classified called as Baltimore Classification

TLS Online TPP Program

#Id: 802

#Unit 3. Fundamental Processes

According to Baltimore, for transcription antisense (-) strand of genome is required

TLS Online TPP Program

#Id: 803

#Unit 3. Fundamental Processes

There are two factors that regulate Prokaryotic Transcription

1. RNA Polymerase

2. Promoter Location & Sequences