Nurturing Life Sciences
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#Unit 13. Methods in Biology
Commonly used heavy metals
Uranyl acetate
Osmium tetraoxide
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Metal shadowing- thin film of platinum is coated from a fixed angle. it provides information about the three-dimensional topology of the sample.
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Scanning electron microscopy (SEM) allows investigators to view the surfaces of unsectioned metal-coated specimens
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Molecules in the metal coating are excited and release secondary electrons that are focused onto a scintillation detector
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To make the antibody visible in the electron microscope, it must be attached to an electron-dense marker.