This method has been used to generate models of ribosomes, the muscle calcium pump, and 3-D structure of a poliovirus capsid.

TLS Online TPP Program

#Id: 2820

#Unit 13. Methods in Biology

Ion-exchange chromatography is frequently chosen for the separation and purification of proteins, peptides, nucleic acids, polynucleotides and other charged molecules, mainly because of its high resolving power and high capacity.

TLS Online TPP Program

#Id: 2821

#Unit 13. Methods in Biology

In Ion-exchange chromatography There are two types of ion exchanger, namely cation  and anion exchangers.

TLS Online TPP Program

#Id: 2822

#Unit 13. Methods in Biology

In Ion-exchange chromatography Cation exchangers possess negatively charged groups and these will attract positively charged cations & Anion exchangers have positively charged groups that will attract negatively charged anions

TLS Online TPP Program

#Id: 2823

#Unit 13. Methods in Biology

Examples of commonly used ion exchangers

TLS Online TPP Program

#Id: 2824

#Unit 13. Methods in Biology

Ion exchangers often use Gradient elution than isocratic elution, while Gradient elution is based on gradient of pH and ionic strength.

TLS Online TPP Program

#Id: 2825

#Unit 13. Methods in Biology

Affinity chromatography exploits the unique property of extremely specific biological interactions to achieve separation and purification.