DIC microscopy easily defines the outlines of large organelles, such as the nucleus and vacuole.

TLS Online TPP Program

#Id: 6452

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6453

#Unit 3. Fundamental Processes

Liquid chromatography separates and identifies the three eukaryotic RNA polymerases, each with its own sensitivity to 𝛂-amanitin.

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#Id: 6454

#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits, Rpb1 and Rpb2

a DNA template, whereas in eukaryotes, transcription takes place on a chromatin template.

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#Unit 3. Fundamental Processes

Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using DEAE-Sephadex ion-exchange chromatography

TLS Online TPP Program

#Id: 6456

#Unit 3. Fundamental Processes

RNA Polymerase can also separated by using epitope tagging. Normally RPB3 is used to create epitope tag.

TLS Online TPP Program

#Id: 6457

#Unit 3. Fundamental Processes

Molecular genetic techniques are used to make a cDNA encoding a recombinant protein to which is fused a short sequence of amino acids called an epitope tagging.