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Caspases western blotting assay is also used to detect apoptosis

#Unit 13. Methods in Biology #Immunotechniques #Part B Pointers
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#Unit 4. Cell Communication and Cell Signaling

ZnA and ZnB on RAG1 are regions of the proteins that form zinc fingers, elongated protein domains stabilized by the coordination of a Zn ion.

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#Unit 4. Cell Communication and Cell Signaling

The ZnA region of the protein assists in the initial RAG1 binding to active chromatin via its ability to interact with histone H3.

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#Unit 4. Cell Communication and Cell Signaling

Granulocytes
1. Neutrophils
2. Basophils
3. Mast cells
4. Eosinophils

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#Unit 4. Cell Communication and Cell Signaling

Granulocytes can be seen by FACS.


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#Unit 4. Cell Communication and Cell Signaling

Neutrophils constitute the majority (50% to 70%) of circulating leukocytes Eosinophils (1%–3%), Basophils (1%), or Mast cells (1%).

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#Unit 4. Cell Communication and Cell Signaling

The resulting transient increase in the number of circulating neutrophils, called leukocytosis.

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