Nurturing Life Sciences
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Some cells carry a cheater mutation that makes spores but that avoids contributing to the stalk.
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#Unit 13. Methods in Biology
Isoschizomers are applicable in recognizing methylation sites genomes.
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Two REs recognize the same DNA sequence motif but cleave at different positions, then they are called neoschizomers
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Process of replication in which gap is created by DNase I, filled by klenow fragment and with radioactive dNTPs called as Nick Translation
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Terminal transferase (TdT) Adds homopolymer tails to the 3’end of DNA.
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T4 DNA ligase Links 5-phosphate and 3-hydroxyl ends via phosphodiester bond.
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T4 polynucleotide kinase (PNK) Transfers terminal phosphate groups from ATP to 5-OH groups.