TLS Online TPP Program

#Id: 4697


Degree of genetic relatedness


#Unit 11. Evolution and Behavior #Altruism and Evolution-Group Selection #Part B Pointers
More Pointers
TLS Online TPP Program

#Id: 2491

#Unit 13. Methods in Biology

SDS–PAGE are firstly boiled for 5 min in sample buffer containing - mercaptoethanol or dithiothreitol ( DTT) and SDS. Mercaptoethanol or DTT reduce any disulfide bridges


TLS Online TPP Program

#Id: 2492

#Unit 13. Methods in Biology

In conventional discontinuous gel electrophoresis two type of GEL system is used on top with comb there is a stacking gel with pH 6.8 and below resolving with gel pH 8.8 phenomenon known as isotachophoresis.


TLS Online TPP Program

#Id: 2493

#Unit 13. Methods in Biology

The band-sharpening effect relies on the fact that negatively charged glycinate ions (in the electrophoresis buffer) have a lower electrophoretic mobility than do the protein–SDS complexes, which, in turn, have lower mobility than the chloride ions (Cl − ) of the loading buffer and the stacking gel buffer. 

[Cl−] > [protein–SDS] > [glycinate].


TLS Online TPP Program

#Id: 2494

#Unit 13. Methods in Biology

More recently Different from conventional gels, the matrix of single gel system introduced  which contains  three amino acids (serine, glycine and asparagine).


TLS Online TPP Program

#Id: 2495

#Unit 13. Methods in Biology

The relationship between acrylamide gel concentration and protein fractionation range





TLS Online TPP Program

#Id: 2496

#Unit 13. Methods in Biology

.In the Native (Buffer) Gels electrophoresis SDS is absent, and the proteins are not denatured prior to loading. Since all the proteins in the sample being analyzed carry their native charge at the pH of the gel (normally pH 8.7), proteins separate according to their different electrophoretic mobilities and the sieving effects of the gel.