A number of amino acids (Asp, Glu, Asn, Gln, Arg and His) have weak electronic transitions at around 210 nm. Usually, these cannot be observed in proteins because

they are masked by the more intense peptide bond absorption.

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#Unit 13. Methods in Biology

HOT START PCR were devised to prevent non – specific amplification at low temperature.

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#Unit 13. Methods in Biology

FRET is possible only when the distance between donor and acceptor are within 1 to 10 Å

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#Unit 13. Methods in Biology

GFP chromophore comprises an oxidized form of the tripeptide –Ser65–Tyr66–Gly67–.

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#Unit 13. Methods in Biology

The efficiency of FRET is inversely proportional to the sixth power of the distance between donor and acceptor.

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#Unit 13. Methods in Biology

Short tandem repeats (STRs) are short repeated sequences of DNA (1–10 bp) that account for approximately  3% of the human genome.

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#Unit 13. Methods in Biology

AAAAAAAAAAAAAAA (a 1-base repeat) and CACACACACACACACACACACA (a 2-basen repeating), CAGCAGCAGCAGCAGCAG (a 3 base repeating) are most common.