DnaA is an ATP-binding protein.

The first stage in initiation is binding of the DnaA-ATP protein complex to the fully methylated oriC sequence.

This takes place in association with the inner membrane. DnaA is in the active form only when bound to ATP.

DnaA has intrinsic ATPase activity that hydrolyzes ATP to ADP and thus inactivates itself when the initiation stage ends.

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#Unit 3. Fundamental Processes

The E. coli oriC contains 11 copies of the GATC sequence

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#Unit 3. Fundamental Processes

GATC is a target for methylation at the N6 position of A by Dam Methylase enzyme which uses SAM as methyl group donor

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#Unit 3. Fundamental Processes

Several of methylation sites overlap dnaA boxes

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#Unit 3. Fundamental Processes

DnaA is an ATP-binding protein and its binding to DNA is affected depending on whether ATP

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#Unit 3. Fundamental Processes

DnaA is the E. coli licensing factor that must be made anew for each round of replication.

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#Unit 3. Fundamental Processes

Replication in dam- E. coli depends on its state of methylation.