DnaA is an ATP-binding protein.

The first stage in initiation is binding of the DnaA-ATP protein complex to the fully methylated oriC sequence.

This takes place in association with the inner membrane. DnaA is in the active form only when bound to ATP.

DnaA has intrinsic ATPase activity that hydrolyzes ATP to ADP and thus inactivates itself when the initiation stage ends.

TLS Online TPP Program

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Presence of the s-subunit permitted recognition of authentic RNA polymerase binding sites

TLS Online TPP Program

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#Unit 3. Fundamental Processes

Polymerase binding sites are called promoters

TLS Online TPP Program

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Transcription that begins at promoters is specific, directed by the s-subunit

TLS Online TPP Program

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How tightly does core enzyme v. holoenzyme bind DNA?

TLS Online TPP Program

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#Unit 3. Fundamental Processes

The effect of temperature on the dissociation of the polymerase–T7 DNA complex. 

TLS Online TPP Program

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#Unit 3. Fundamental Processes

higher temperature favors tighter binding between RNA polymerase holoenzyme and T7 DNA