PPDK-RP make PPDK active removing phosphates of catalytic his/threo residues using ADP as phosphoryl donor, rather than ATP, and the phosphorolytic dephosphorylation of the (PPDK)−Thr−O−P with formation of pyrophosphate, rather than the hydrolysis employed by most protein

phosphatases.

TLS Online TPP Program

#Id: 4611

#Unit 13. Methods in Biology

FITC binds to Annexin V to phosphatidyl serine which are found on apoptotic cells, analyzed by FACS

TLS Online TPP Program

#Id: 4612

#Unit 13. Methods in Biology

The TUNEL Assay measures Apoptotically Generated DNA Fragmentation.

TLS Online TPP Program

#Id: 4613

#Unit 13. Methods in Biology

TUNEL method uses TdT to add BrdU to fixed and permeabilized cells which can only incorporated into fragmented DNA of apoptotic cells.

TLS Online TPP Program

#Id: 4614

#Unit 13. Methods in Biology

BrdU is incorporated into the newly synthesized DNA, and is then detected with fluorescently labeled anti-BrdU antibodies

TLS Online TPP Program

#Id: 4615

#Unit 13. Methods in Biology

Caspases western blotting assay is also used to detect apoptosis

TLS Online TPP Program

#Id: 4616

#Unit 13. Methods in Biology

The total concentration of antibody in the equilibrium dialysis chamber is known, the equilibrium equation can be rewritten as: