Nurturing Life Sciences
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#Unit 2. Cellular Organization
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#Unit 4. Cell Communication and Cell Signaling
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#Unit 13. Methods in Biology
Primer sequence also· affects PCR efficiency. For example, if the primers used for PCR have complementary regions, primer dimers will be formed, and PCR efficiency will markedly decline.
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Touchdown PCR is initiated at very high annealing temperatures, which allow only perfectly matched primer-template DNA hybrids to form and support amplification. The annealing temperature is dropped in a stepwise fashion with each cycle of PCR.
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RII phosphorylates serine and threonine in the juxtamembrane segment of the RI (ligand induced receptor hetero-oligomerization), releasing the inhibition of RI kinase activity.