Nurturing Life Sciences
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#Unit 3. Fundamental Processes
The E. coli oriC is fully methylated at the beginning of replication.
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oriC is hemimethylated and remains in that condition for approximately 13 minutes.
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SeqA binds to hemimethylated DNA more strongly than to fully methylated DNA.
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Deletion of dat causes initiation to occur more frequently. This significantly increases the amount of DnaA available to the origin.
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DnaA is provided by its response to phospholipids. Phospholipids promote the exchange of ATP with ADP bound to DnaA.
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While DnaA has a weak intrinsic activity that converts the ATP to ADP, this is enhanced by a factor termed Hda.