All prokaryotic and eukaryotic DNA polymerases share the same fundamental type of synthetic activity -an antiparallel synthesis from 5’ to 3’ from a template that is 3’ to 5’. This means adding nucleotides one at a time to a 3’–OH end.

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#Unit 4. Cell Communication and Cell Signaling

Junctional diversity- P and N nucleotides  addition 

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#Unit 4. Cell Communication and Cell Signaling

P nucleotide addition results when the DNA hairpin at the coding joint is cleaved asymmetrically.

TLS Online TPP Program

#Id: 7491

#Unit 4. Cell Communication and Cell Signaling

Non - templated N nucleotide addition in heavy chains results from TdT activity. 

TLS Online TPP Program

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#Unit 4. Cell Communication and Cell Signaling

Exonuclease trimming sometimes occurs at the VDJ and VJ junctions, causing loss of nucleotides.

TLS Online TPP Program

#Id: 7493

#Unit 4. Cell Communication and Cell Signaling

Combinatorial diversity: The same heavy chain can combine with different light chain

TLS Online TPP Program

#Id: 7494

#Unit 4. Cell Communication and Cell Signaling