DNA polymerases fall into five families based on sequence homologies; the palm is well conserved among them, but the thumb and fingers provide analogous secondary structure elements from different sequences.

TLS Online TPP Program

#Id: 8544

#Unit 1. Structure and Function of Biomolecules

The abscissa refers to the (x) coordinate and the ordinate refers to the (y) coordinate of a standard two-dimensional graph.

TLS Online TPP Program

#Id: 8545

#Unit 1. Structure and Function of Biomolecules

Irreversible inhibitors, such as the organophosphorus and organomercury compounds, cyanide, carbon monoxide and hydrogen sulphide, combine with the enzyme to form a covalent bond. 

TLS Online TPP Program

#Id: 8546

#Unit 1. Structure and Function of Biomolecules

The extent of irreversible inhibition of the enzyme is dependent upon the reaction rate constant (and hence time) for covalent bond formation and upon the amount of inhibitor present.

TLS Online TPP Program

#Id: 8547

#Unit 1. Structure and Function of Biomolecules

The irreversible inhibitors cannot be removed by simple physical techniques such as dialysis, is to reduce the amount of enzyme available for reaction.

TLS Online TPP Program

#Id: 8548

#Unit 1. Structure and Function of Biomolecules

TLS Online TPP Program

#Id: 8549

#Unit 1. Structure and Function of Biomolecules

The irreversible inhibition involves reactions with a functional group, such as hydroxyl or sulphydryl, or with a metal atom in the active site or a distinct allosteric site.