The extent of irreversible inhibition of the enzyme is dependent upon the reaction rate constant (and hence time) for covalent bond formation and upon the amount of inhibitor present.

TLS Online TPP Program

#Id: 8941

#Unit 2. Cellular Organization

Spindle position checkpoint pathway ensures that the nucleus is accurately partitioned between two daughter cells

TLS Online TPP Program

#Id: 8942

#Unit 2. Cellular Organization

In S. cerevisiae, during interphase and early mitosis, Cdc14 is sequestered and inactivated in the nucleolus. 

TLS Online TPP Program

#Id: 8943

#Unit 2. Cellular Organization

Inactive Tem1-GDP associates with the spindle pole body nearest to the bud as soon as the mitotic spindle forms

TLS Online TPP Program

#Id: 8944

#Unit 2. Cellular Organization

If chromosome segregation occurs properly, extension of the spindle microtubules inserts the daughter SPB into the bud, causing Tem1 to be activated by an unknown mechanism.

TLS Online TPP Program

#Id: 8945

#Unit 2. Cellular Organization

Tem1-GTP activates a protein kinase cascade, which promotes the release of active Cdc14 from the nucleolus and exit from mitosis. 

TLS Online TPP Program

#Id: 8946

#Unit 2. Cellular Organization

An inhibitor of the GTPase, called Kin4, localizes to the parent cell but is absent from the bud.