A new model for the structure of the DNA Pol III complex proposes a three-polymerase core structure, with two Pol III catalytic cores responsible for synthesis of the lagging strand and one for the leading strand.

TLS Online TPP Program

#Id: 2898

#Unit 13. Methods in Biology

The denser the biological buffer system is, the slower the particle will move in a

centrifugal field

TLS Online TPP Program

#Id: 2900

#Unit 13. Methods in Biology

The greater the frictional coefficient is, the slower a particle will move

TLS Online TPP Program

#Id: 2901

#Unit 13. Methods in Biology

The greater the centrifugal force is, the faster the particle sediments

TLS Online TPP Program

#Id: 2902

#Unit 13. Methods in Biology

The sedimentation rate of a given particle will be zero when the density of the

particle and the surrounding medium are equal.

TLS Online TPP Program

#Id: 2910

#Unit 13. Methods in Biology

The rate of sedimentation is dependent upon the applied centrifugal field (cm s-2), G,

that is determined by the radial distance, r, of the particle from the axis of rotation (in cm)

and the square of the angular velocity ω, of the rotor (in radians per second):

TLS Online TPP Program

#Id: 2911

#Unit 13. Methods in Biology

Since 3600 equals 2 radians, one revolution of the rotor can be expressed as 2 rad.