E. coli SSB is a tetramer of 74 kD that binds single-stranded DNA cooperatively

TLS Online TPP Program

#Id: 769

#Unit 3. Fundamental Processes

After release of the abortive product, the enzyme again begins synthesizing RNA at position +1 called as Initiation

TLS Online TPP Program

#Id: 770

#Unit 3. Fundamental Processes

Once an enzyme makes a transcript longer than 10 nucleotides, it is said to have escaped the promoter. At this point, it has formed a stable ternary complex, containing enzyme, DNA, and RNA. This is the transition to the elongation phase.

TLS Online TPP Program

#Id: 771

#Unit 3. Fundamental Processes

During RNA transcript formation, DNA makes its 90° turn at the active site.

TLS Online TPP Program

#Id: 772

#Unit 3. Fundamental Processes

In the core polymerase active site, Process of the nucleotide addition cycle and undergoing translocation takes place by a Brownian ratchet mechanism.

 

TLS Online TPP Program

#Id: 773

#Unit 3. Fundamental Processes

DNA polymerase does not synthesize new DNA strands de novo while RNA Polymerase initiates RNA synthesis de novo without primer

TLS Online TPP Program

#Id: 774

#Unit 3. Fundamental Processes

RNA polymerase starts most transcripts with an A (75%) or G