Sphingolipids contain a single fatty acid, in amide linkage to the sphingosine backbone.

TLS Online TPP Program

#Id: 6460

#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell.

Alexa- 488-labeled antibody -emit green light

Alexa-568-labeled antibody- emit red light

TLS Online TPP Program

#Id: 6461

#Unit 3. Fundamental Processes

A significant difference between the transcription of eukaryotic and prokaryotic RNAs is that in bacteria, transcription takes place on  RNA polymerase III synthesizes small RNAs in the nucleoplasm

TLS Online TPP Program

#Id: 6462

#Unit 3. Fundamental Processes

A typical gene transcribed by RNA polymerase II has a promoter that extends upstream from the site where transcription is initiated.

TLS Online TPP Program

#Id: 6463

#Unit 3. Fundamental Processes

A regulatory site that binds more negative regulators than positive regulators to control transcription is called a silencer.

TLS Online TPP Program

#Id: 6464

#Unit 3. Fundamental Processes

Promoters that are constitutively expressed and needed in all cells (their genes are sometimes called housekeeping genes) have upstream sequence elements that are recognized by ubiquitous activators.

TLS Online TPP Program

#Id: 6465

#Unit 3. Fundamental Processes

Proteins bound at enhancer elements interact with proteins bound at promoter elements via DNA looping, very often through intermediates called coactivators.