The holoenzyme is a complex of 900 kD that contains 10 different proteins organized into four types of subcomplex

1. Atleast two copies of the catalytic core.

2. two copies of the dimerizing subunit, t, which link the two catalytic cores together.

3. There are two copies of the clamp

4. The g complex is a group of seven proteins, encoded by five genes, that comprise the clamp loader

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#Unit 3. Fundamental Processes

Several of methylation sites overlap dnaA boxes

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#Unit 3. Fundamental Processes

DnaA is an ATP-binding protein and its binding to DNA is affected depending on whether ATP

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#Unit 3. Fundamental Processes

DnaA is the E. coli licensing factor that must be made anew for each round of replication.

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#Unit 3. Fundamental Processes

Replication in dam- E. coli depends on its state of methylation.

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#Unit 3. Fundamental Processes

In dam- E. coli If the plasmid is methylated, it undergoes a single round of replication, and if it is hemimethylated products accumulate

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#Unit 3. Fundamental Processes

The GATC sites at the origin remain hemimethylated for 13 minutes after replication.