Nurturing Life Sciences
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#Unit 13. Methods in Biology
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Normal-phase liquid chromatography form of partition chromatography
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In Normal-phase liquid chromatography the stationary phase is polar and the mobile phase relatively non-polar.
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In NPLC the most popular stationary phase is an alkylamine bonded to silica. The mobile phase is generally an organic solvent such as hexane, heptane, dichloromethane or ethyl acetate.
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In NPLC solvents form an elutropic series based on their polarity. Such a series in
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