How to calculate linking number?

TLS Online TPP Program

#Id: 6401

#Unit 2. Cellular Organization

Attenuator riboswitch (Leader sequence) is made up of almost 140 nt which further can be divided into four Intra -complementary regions 

TLS Online TPP Program

#Id: 6402

#Unit 2. Cellular Organization

Attenuator riboswitch (Leader sequence) contains two major check-points in region 1

UGG codons on position 10, 11

A stop codon UGA between Region 1 and 2

TLS Online TPP Program

#Id: 6403

#Unit 2. Cellular Organization

Attenuator riboswitch (Leader sequence) region 1 is translated atleast till codon 10 and 11 in case of Low trp concentration

TLS Online TPP Program

#Id: 6404

#Unit 2. Cellular Organization

Region 1 Leader peptide is of 14 Amino acids Met------Ser 

TLS Online TPP Program

#Id: 6405

#Unit 2. Cellular Organization

At high or low trp concentration, RNA Polymerase synthesizes full mRNA till region 4

TLS Online TPP Program

#Id: 6406

#Unit 2. Cellular Organization

Attenuator riboswitch (Leader sequence) region 1 is translated  till UGA (R1-2) at high trp concentration due to two conditions

 Ribosome quickly crosses codon 10, 11

After crossing codon 10, 11 ribosome prematurely terminated at UGA at the end of Region 1