TLS Online TPP Program

#Id: 2889

#Unit 3. Fundamental Processes

Drugs that block protein synthesis block a new round of replication, but not continuation of replication.

TLS Online TPP Program

#Id: 2890

#Unit 3. Fundamental Processes

Initiation of replication at oriC starts with formation of a complex that ultimately requires six proteins: DnaA, DnaB, DnaC, HU, gyrase, and SSB.

TLS Online TPP Program

#Id: 2891

#Unit 3. Fundamental Processes

the origin has been opened (and the DNA is single-stranded) by its ability to further unwind the DNA.

TLS Online TPP Program

#Id: 2892

#Unit 3. Fundamental Processes

DnaA is an ATP-binding protein. The first stage in initiation is binding of the DnaA-ATP 

protein complex to the fully methylated oriC sequence.

TLS Online TPP Program

#Id: 2893

#Unit 3. Fundamental Processes

DnaA is in the active form only when bound to ATP.

TLS Online TPP Program

#Id: 2894

#Unit 3. Fundamental Processes

Dna A has intrinsic ATPase activity that hydrolyzes ATP to ADP and thus inactivates itself when the initiation stage ends.