Orc2-5 binds strongly; Orc6 binds weakly to ARS and has a nuclear localization signal that must be activated by the cyclin/ CDK kinase during the G1 to S transition.

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#Id: 4600

#Unit 13. Methods in Biology

BrdU can CAUSE light induced cell death

TLS Online TPP Program

#Id: 4601

#Unit 13. Methods in Biology

If cells that have incorporated a high level of BrdU are exposed to light, they will photolyse; this has been used to selectively kill newly dividing cells.

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#Id: 4602

#Unit 13. Methods in Biology

Propidium iodide is a fluorescent dye with a flat, planar structure that slides between the rungs of (or intercalates into) the DNA ladder in a quantitative manner frequently used in FACS.

TLS Online TPP Program

#Id: 4603

#Unit 13. Methods in Biology

Propidium iodide-labeled cells can be identify cells within the populations are at each stage of the cell cycle

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#Id: 4604

#Unit 13. Methods in Biology

 Propidium iodide-labeled cells in order as
1. Apoptotic cells
2. G1/G0
3. S
4. G2/M 

TLS Online TPP Program

#Id: 4605

#Unit 13. Methods in Biology

Other dyes that bind to DNA and allow for similar types of cell cycle analysis include DAPI, Hoechst 33342, and 7-aminoactinomycin D (AAD).