The presence of Cdc6 and Cdt1 at the yeast origin allows the MCM proteins to bind to the complex in G1 in the inactive state.

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#Id: 2507

#Unit 13. Methods in Biology

Two-Dimensional Polyacrylamide Gel Electrophoresis  technique combines the technique of IEF (first dimension), which separates proteins in a mixture according to charge (pI), with the size separation technique of SDS–PAGE (second dimension).

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#Id: 2508

#Unit 13. Methods in Biology

Cellulose Acetate Electrophoresis have clinical role for example enzyme and antibodies are easily detected by using Cellulose Acetate Electrophoresis with zymogen technique.

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#Id: 2509

#Unit 13. Methods in Biology

The most commonly used general protein stain for detecting protein on gels is the sulfated trimethylamine dye Coomassie Brilliant Blue R-250 (CBB).

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#Id: 2510

#Unit 13. Methods in Biology

Silver staining gives higher sensitivity comparison to Coomassie Brilliant Blue (CBB).

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#Id: 2511

#Unit 13. Methods in Biology

Glycoproteins have traditionally been detected on protein gels by use of the periodic acid–Schiff ( PAS) stain

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#Id: 2512

#Unit 13. Methods in Biology

Quantitative analysis (i.e., measurements of the relative amounts of different proteins in a sample) can be achieved by scanning densitometry .