lacZ encodes for b-galactosidase,a tetramer of 500 kD. The enzyme breaks lactose is cleaved into glucose and galactose.  This enzyme also produces an important byproduct, b-1,6-allolactase.

TLS Online TPP Program

#Id: 4049

#Unit 2. Cellular Organization

This is used to identify when regulatory proteins bound at an enhancer are in close proximity to the transcriptional machinery at a given promoter—a physical proximity interpreted to show looping out of the DNA

TLS Online TPP Program

#Id: 4050

#Unit 2. Cellular Organization

The interphase nuclei of some tissues of the larvae of dipteran flies contain chromosomes that are greatly enlarged relative to their usual condition. They possess both increased diameter and greater length. shows an example of a chromosome set from the salivary gland of D. melanogaster. The members of this set are called polytene chromosomes.

TLS Online TPP Program

#Id: 4051

#Unit 2. Cellular Organization

The centromeres of all four chromosomes of D. melanogaster aggregate to form a chromocenter that consists largely of heterochromatin.

TLS Online TPP Program

#Id: 4052

#Unit 2. Cellular Organization

Each is produced by the successive replications of a synapsed diploid pair of chromosomes. The replicas do not separate, but instead remain attached to each other in their extended state, a process known as endoreduplication.

At the start of the process, each synapsed pair has a DNA content of 2C (where C represents the DNA content of the individual chromosome). This amount then doubles up to nine times, at its maximum giving a content of 1024C. The number of doublings is different in the various tissues of the D. melanogaster larva.

TLS Online TPP Program

#Id: 6359

#Unit 2. Cellular Organization

Operon consist

Physiology/Mechanism

Molecular biology/Biochemistry of component

Effect of mutations

TLS Online TPP Program

#Id: 6360

#Unit 2. Cellular Organization

Repressors impede access of RNA polymerase to the promoter and Activators enhance the RNA polymerase–promoter interaction.