Nurturing Life Sciences
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IPTG (isopropyl b-D-1-thiogalactopyranoside)
X-gal (5-bromo4-chloro-3-indolyl-b-D-galactopyranoside). When acted on by that enzyme, X-gal releases a blue color that makes it useful as substrate in assays.
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#Unit 3. Fundamental Processes
Su(var) mutations lie in genes whose products are needed for the formation of heterochromatin such as HDAC, HMT.
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E(var) mutations lie in genes whose products are needed to activate gene expression. They include members of the SWI/SNF complex
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Mutation of a deacetylase that acts on H3K14Ac prevents the methylation at K9 because HDAC provide methylation sites for HMT.
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Methylation of H3K9 demarcates heterochromatin while H3K4 methylation demarcates euchromatin.
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Heterochromatin formation at telomeres and silent mating-type loci in yeast relies on an overlapping set of genes known as silent information regulators (SIR genes).