IPTG (isopropyl b-D-1-thiogalactopyranoside) 

X-gal (5-bromo4-chloro-3-indolyl-b-D-galactopyranoside). When acted on by that enzyme, X-gal releases a blue color that makes it useful as substrate in assays.

TLS Online TPP Program

#Id: 6669

#Unit 3. Fundamental Processes

There are three type of Splicing signals 

 Major U2-type or GU-AG introns

Minor U12-type or AU-AC introns

Minor U12-type with GU-AG introns (hybrid type)

TLS Online TPP Program

#Id: 6670

#Unit 3. Fundamental Processes

UACUAAC is branch point sequence for U2 type introns CCUUAAC is branch point sequence for U12 type introns

TLS Online TPP Program

#Id: 6671

#Unit 3. Fundamental Processes

Major U2-type or GU-AG introns

TLS Online TPP Program

#Id: 6672

#Unit 3. Fundamental Processes

Minor U12-type with GU-AG introns (hybrid type)

TLS Online TPP Program

#Id: 6673

#Unit 3. Fundamental Processes

Splice sites are directional in nature. 5’ splice site -the left, or donor, site and the 3’ splice site - right, or acceptor, site

TLS Online TPP Program

#Id: 6674

#Unit 3. Fundamental Processes