Splicing is an SN2 reaction that proceeds through a pentavalent phosphorous intermediate.

TLS Online TPP Program

#Id: 6771

#Unit 3. Fundamental Processes

nonstop-mediated decay rescues ribosomes that translate mRNAs that lack a stop codon. When an mRNA lacking a stop codon is translated, the ribosome translates through the poly-A tail This results 

TLS Online TPP Program

#Id: 6772

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6773

#Unit 3. Fundamental Processes

The cap binding CBP20/80 complex appears to directly bind to the mRNA export machinery (the TREX complex)

TLS Online TPP Program

#Id: 6774

#Unit 3. Fundamental Processes

(NXF1), (NXT1), SR proteins, RNA helicases, REF (RNA export factor), PABPN1 are important proteins required for mRNA transport.

TLS Online TPP Program

#Id: 6775

#Unit 3. Fundamental Processes

mRNP export from the nucleus into the cytoplasm is controlled by the phosphorylation and dephosphorylation of mRNP adapter proteins, REF, the NXF1/NXT1 mRNP exporter to mRNPs. 

TLS Online TPP Program

#Id: 6776

#Unit 3. Fundamental Processes

SR Proteins, in its phosphorylated form, the SR protein initially binds to nascent pre-mRNA. When 3′ cleavage and polyadenylation are completed, the adapter protein is dephosphorylated by a specific nuclear protein phosphatase that is essential for mRNP export. Only the dephosphorylated adapter protein can bind the mRNP exporter, thereby coupling mRNP export to correct polyadenylation.