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TLS Online TPP Program

#Id: 6810

RNA-dependent RNA polymerase (RdRP), an additional enzyme required in many cases of RNAi including centromeric silencing in fission yeast.



#Unit 3. Fundamental Processes #mRNA stability, transport, CRISPR, miRNA & siRNA #Part B Pointers
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TLS Online TPP Program

#Id: 5996

#Unit 3. Fundamental Processes
TLS Online TPP Program

#Id: 5997

#Unit 3. Fundamental Processes
TLS Online TPP Program

#Id: 5998

#Unit 3. Fundamental Processes

An ARS element consists of an A-T–rich region that contains discrete sites in which mutations affect origin function.
TLS Online TPP Program

#Id: 5999

#Unit 3. Fundamental Processes

A 14-bp “core” region, called the A domain, which contains an 11-bp consensus sequence consisting of A-T base pairs. This consensus sequence (sometimes called the ACS, for ARS)
TLS Online TPP Program

#Id: 6000

#Unit 3. Fundamental Processes

The origin recognition complex (ORC) is a highly conserved complex found in all eukaryotes.  It is composed of six proteins with a mass of 400 kD.  ORC binds to the yeast A and B1 elements on the A-T–rich strand and is associated with ARS elements throughout the cell cycle.
TLS Online TPP Program

#Id: 6001

#Unit 3. Fundamental Processes

By counting the number of sites to which ORC binds, we can estimate that there are about 400 origins of replication in the yeast genome. This means that the average length of a replicon is 35,000 bp.