Nurturing Life Sciences
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#Unit 13. Methods in Biology
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DNA gives absorbance at 595 nm in presence of Diphenylamine while gives direct absorbance at 260 nm
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RNA gives absorbance at 595 nm in presence of Diphenylamine while gives direct absorbance at 540 nm.
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The electronic transitions of the peptide bond occur in the far UV. The intense peak at 190 nm, and the weaker one at 210–220 nm is due to the p ® p* and n ® p* transitions.