In D. discoideum cAMP phosphodiesterase RegA plays a crucial role in regulating intracellular cAMP levels cells & level of PKA activity.

TLS Online TPP Program

#Id: 4461

#Unit 13. Methods in Biology

Scanned-probe microscope scans the specimen without modifying it or exposing it to damaging high -energy radiation. 

TLS Online TPP Program

#Id: 4462

#Unit 13. Methods in Biology

Scanned-probe microscopy is of two types:

Scanning tunneling microscopy

Atomic force microscopy

TLS Online TPP Program

#Id: 4463

#Unit 13. Methods in Biology

Scanning tunneling microscopy- uses thin metal (tungsten) probe that scans specimen revealing the bumps and depressions of the atoms on the surface of the specimen

TLS Online TPP Program

#Id: 4464

#Unit 13. Methods in Biology

In an atomic force microscope imaging of the surface is done based on modulation of interaction forces between two atoms.

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#Id: 4465

#Unit 13. Methods in Biology

Cryoelectron microscopy allows examination of hydrated, unfixed, and unstained specimens in the TEM by maintaining them at -196oC (liquid nitrogen).

TLS Online TPP Program

#Id: 4466

#Unit 13. Methods in Biology

This method has been used to generate models of ribosomes, the muscle calcium pump, and 3-D structure of a poliovirus capsid.