Once a sperm has reached the oocyte-cumulus complex, hyperactivation, along with a hyaluronidase enzyme on the outside of the sperm cell membrane, enables the sperm to digest a path through the extracellular matrix of the cumulus cells

TLS Online TPP Program

#Id: 889

#Unit 13. Methods in Biology

A recognition site frequency every 44 (256) bp, 46 (4096) bp and 48 (65 536) bp, if A=T=G=C.

TLS Online TPP Program

#Id: 890

#Unit 13. Methods in Biology

Frequency of recognition site depends on A/T::G/C ratio of genome.

TLS Online TPP Program

#Id: 891

#Unit 13. Methods in Biology

The nuclear genome of mammals is 40% G+C and the dinucleotide CG is fivefold less common than statistically expected.

TLS Online TPP Program

#Id: 892

#Unit 13. Methods in Biology

CCG and CGG are the rarest trinucleotides in most A+T-rich bacterial genomes and CTAG is the rarest tetranucleotide in G+C-rich bacterial genomes.

TLS Online TPP Program

#Id: 893

#Unit 13. Methods in Biology

RE’ has two functions-

a) Restriction of foreign genome

b) Modification/methylation of host genome using SAM

TLS Online TPP Program

#Id: 894

#Unit 13. Methods in Biology

Recognize the same sequence and cut at exactly the same position, these enzymes are called isoschizomers.