Transport of proteins from the cytoplasm into the nucleus and movement of macromolecules, including mRNAs, tRNAs, and ribosomal subunits, out of the nucleus occurs through nuclear pores, which span both membranes of the nuclear envelope.

#Id: 732

#Unit 3. Fundamental Processes

The GATC sites at the origin remain hemimethylated for 13 minutes after replication.

#Id: 733

#Unit 3. Fundamental Processes

DNA methylation in bacteria allows the DNA mismatch recognition machinery to distinguish the old template strand from the new strand.

#Id: 734

#Unit 3. Fundamental Processes

Initiation of replication in E.Coli at oriC starts with formation of a complex that ultimately requires six proteins: DnaA, DnaB, DnaC, HU, gyrase, and SSB

#Id: 735

#Unit 3. Fundamental Processes

The minimal origin is defined by the distance between the outside members of the 13-mer and 9-mer repeats.

#Id: 736

#Unit 3. Fundamental Processes

The four 9-bp consensus sequences on the right side of oriC provide the initial binding sites for DnaA-ATP

#Id: 737

#Unit 3. Fundamental Processes

A two-state assembly model explains melting of 13mer during initiation