All proteins found in the nucleus, histones, transcription factors,  DNA & RNA polymerases are synthesized in the cytoplasm and imported into the nucleus through nuclear pore complexes.

TLS Online TPP Program

#Id: 799

#Unit 3. Fundamental Processes

Mutations in the rpoB gene can reduce termination at a rho-dependent site.  Mutations in rpoB can restore the ability to terminate transcription at rho-dependent sites in rho mutant bacteria.

TLS Online TPP Program

#Id: 800

#Unit 3. Fundamental Processes

Transcription is formation of RNA from genome (DNA/RNA)

TLS Online TPP Program

#Id: 801

#Unit 3. Fundamental Processes

On the basis of Transcription, viruses have been classified called as Baltimore Classification

TLS Online TPP Program

#Id: 802

#Unit 3. Fundamental Processes

According to Baltimore, for transcription antisense (-) strand of genome is required

TLS Online TPP Program

#Id: 803

#Unit 3. Fundamental Processes

There are two factors that regulate Prokaryotic Transcription

1. RNA Polymerase

2. Promoter Location & Sequences

TLS Online TPP Program

#Id: 804

#Unit 3. Fundamental Processes

RNA Polymerase SDS page shows two very large subunits: beta (b) and beta-prime (b’), with molecular masses of 150 and 160 kD, respectively.