NLSs were first discovered through the analysis of mutations of the gene for large T-antigen encoded by simian virus 40 (SV40).

TLS Online TPP Program

#Id: 8563

#Unit 1. Molecules and their Interaction Relevant to Biology

Substrate-binding could cause a conformational change to take place in the enzyme and reveal an inhibitor binding site, or the inhibitor could bind directly to the enzyme-bound substrate. 

TLS Online TPP Program

#Id: 8564

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8565

#Unit 1. Molecules and their Interaction Relevant to Biology

In the simplest possible model, simple linear non-competitive inhibition, the substrate does not affect inhibitor-binding. 

TLS Online TPP Program

#Id: 8566

#Unit 1. Molecules and their Interaction Relevant to Biology

Under these conditions, the reactions have an identical dissociation constant Ki , again called the inhibitor constant. 

TLS Online TPP Program

#Id: 8568

#Unit 1. Molecules and their Interaction Relevant to Biology

In the presence of a non-competitive inhibitor which will bind equally well to E or to ES- Vmax decreases but not Km 

TLS Online TPP Program

#Id: 8569

#Unit 1. Molecules and their Interaction Relevant to Biology

In case of nonidentical dissociation constants, MIXED TYPE INHIBITION TAKES PLACE