Signal sequence

ER targeting sequence, located at the N-terminus of the protein

TLS Online TPP Program

#Id: 2799

#Unit 13. Methods in Biology

Example of a van Deemter plot. The plot shows that the optimum flow rate for a given column is the net result of the influence of flow rate on longitudinal diffusion, equilibration time and multiple pathways.

TLS Online TPP Program

#Id: 2800

#Unit 13. Methods in Biology

A chromatogram is a pictorial record of the detector response as a function of elution volume or retention time.

TLS Online TPP Program

#Id: 2801

#Unit 13. Methods in Biology

Normal-phase liquid chromatography form of partition chromatography

TLS Online TPP Program

#Id: 2802

#Unit 13. Methods in Biology

In Normal-phase liquid chromatography the stationary phase is polar and the mobile phase relatively non-polar.

TLS Online TPP Program

#Id: 2803

#Unit 13. Methods in Biology

In NPLC the most popular stationary phase is an alkylamine bonded to silica. The mobile phase is generally an organic solvent such as hexane, heptane, dichloromethane or ethyl acetate.

TLS Online TPP Program

#Id: 2804

#Unit 13. Methods in Biology

In NPLC solvents form an elutropic series based on their polarity. Such a series in