O-linked oligosaccharides

Carbohydrate chains in glycoproteins attached to the

hydroxyl group (-OH) in serine and threonine residues

TLS Online TPP Program

#Id: 3124

#Unit 3. Fundamental Processes

There is a 13-minute delay before the GATC/CTAG repeats are remethylated. 

TLS Online TPP Program

#Id: 3126

#Unit 3. Fundamental Processes

oriC contains binding sites for DnaA: dnaA-boxes.DnaA is an ATP-bindingprotein and its binding to DNA is affected depending on whether ATP, ADP, or no nucleotide is bound.

TLS Online TPP Program

#Id: 3127

#Unit 3. Fundamental Processes

One mechanism by which the activity of the replication origin is controlled is DNA methylation. 

TLS Online TPP Program

#Id: 3128

#Unit 3. Fundamental Processes

The E. coli oriC contains 11 copies of the sequence GATC/CTAG which is a target for methylation at the N6 position of adenine by the Dam methylase enzyme.

TLS Online TPP Program

#Id: 3129

#Unit 3. Fundamental Processes

Before replication, the palindromic target site is methylated on the adenines of each strand.

TLS Online TPP Program

#Id: 3130

#Unit 3. Fundamental Processes

Replication inserts the normal (nonmodified) bases into the daughter strands. This generates hemimethylated DNA, in which one strand is methylated and one strand is unmethylated.