The differentiation of niches that enables similar species to coexist in a community is called resource partitioning.

TLS Online TPP Program

#Id: 2531

#Unit 13. Methods in Biology

Primers with significant secondary structure are undesirable.

TLS Online TPP Program

#Id: 2532

#Unit 13. Methods in Biology

In PCR, there should be no complementarity between the two primers. 

TLS Online TPP Program

#Id: 2533

#Unit 13. Methods in Biology

The ‘Wallace rule’ (Lay Thein & Wallace 1986) is used to determine the appropriate stringency wash temperature: 

Tm = 4 × (number of GC base pairs) + 2 × (number of AT base pairs

TLS Online TPP Program

#Id: 2534

#Unit 13. Methods in Biology

Probe length for PCR – the longer the oligonucleotide, the less chance there is of it binding to sequences other than the desired target sequence under conditions of high stringency.

TLS Online TPP Program

#Id: 2535

#Unit 13. Methods in Biology

Oligonucleotide composition for PCR– the GC content will influence the stability of the resultant hybrid and hence the determination of the appropriate stringency washing conditions

. 

TLS Online TPP Program

#Id: 2536

#Unit 13. Methods in Biology

At the second cycle of PCR, primers will anneal to the 'long product' at sites where sequences complementary· to them are located, which will be much before the actual 3'-end of the long product.