Negatively charged amino acids have four ionisations zones

1. 1--------pK1 – charge +1

2. pK1---------pKr charge 0

3. pKr---------pK2 charge -1

4. pK2---------14 charge -2

TLS Online TPP Program

#Id: 6467

#Unit 3. Fundamental Processes

There are five common subunits in all three RNA Polymerases

Rpb5

Rpb6

Rpb8

Rpb10

Rpb12

TLS Online TPP Program

#Id: 6468

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6469

#Unit 3. Fundamental Processes

There are 27 of these repeats in the yeast Pol II CTD, 32 in the worm Caenorhabditis elegans, 45 in the fly Drosophila, and 52 in humans.  YSPTSPS repeats in CTD corresponds to the complexity of the genome.

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#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 6471

#Unit 3. Fundamental Processes

The RNA polymerase I promoter consists of a core promoter and an upstream promoter element (UPE).

TLS Online TPP Program

#Id: 6472

#Unit 3. Fundamental Processes

RNA polymerase I requires two ancillary transcription factors to recognize the promoter sequence. The factor that binds to the core promoter is SL1 which consists of four protein subunits. SL1 has primary responsibility for RNA polymerase recruitment