The holoenzyme could transcribe intact phage T4 DNA in vitro quite actively, the core enzyme had little ability to do this.

TLS Online TPP Program

#Id: 8320

#Unit 2. Cellular Organization

The lag phase can be reduced or abolished entirely by adding premade nuclei.

TLS Online TPP Program

#Id: 8321

#Unit 2. Cellular Organization

Elongation phase

short oligomer rapidly increases in length by the addition of actin monomers to both of its ends, causing filament elongation

TLS Online TPP Program

#Id: 8322

#Unit 2. Cellular Organization

Steady-state phase

G-actin monomers exchange with subunits at the filament ends, but there is no net change in the total length of filaments.

TLS Online TPP Program

#Id: 8323

#Unit 2. Cellular Organization

TLS Online TPP Program

#Id: 8324

#Unit 2. Cellular Organization

The rate of addition of monomers is given by

rate constant kon (M–1 sec–1 )

The rate of loss is given by koff (sec–1)

TLS Online TPP Program

#Id: 8325

#Unit 2. Cellular Organization

Critical concentration (Cc)

minimal concentration of monomers at which filaments form