For optimal activity, of acid hydrolases they need to be: Activated by proteolytic cleavage Requires an acid environment.

TLS Online TPP Program

#Id: 6312

#Unit 3. Fundamental Processes

TRCF has an ATPase activity. 

Base-pairing of something to the transcript to destabilize RNA-DNA hybrid

Causes hairpin to form

Causing the transcription to pause

Causes a string of U’s to be incorporated just downstream of hairpin

TLS Online TPP Program

#Id: 6313

#Unit 3. Fundamental Processes

Sequence of a Rho-independent terminator

TLS Online TPP Program

#Id: 6314

#Unit 3. Fundamental Processes

There are 1100 sequences terminators in the E. coli genome

Intrinsic terminators include palindromic regions that form hairpins varying in length from 7 to 20 bp. The stem-loop structure includes a G-C–rich region and is followed by a run of 7U residues.

TLS Online TPP Program

#Id: 6315

#Unit 3. Fundamental Processes

Model of Intrinsic Termination

Bacterial terminators act by:

TLS Online TPP Program

#Id: 6316

#Unit 3. Fundamental Processes

Readthrough transcripts refer to the fraction of transcripts that are not stopped by the terminator.  Furthermore, the termination event can be prevented by specific ancillary factors that interact with RNA and/or RNA polymerase, a situation referred to as antitermination.

TLS Online TPP Program

#Id: 6317

#Unit 3. Fundamental Processes